目的 基于多元活性成分同时测定结合多元统计分析综合评价不同产地连翘药材的质量。方法 采用超快速液相色谱-三重四极杆/线性离子阱质谱法(UFLC-QTRAP-MS/MS)同时测定不同产地连翘药材中苯乙醇苷、木脂素、黄酮及酚酸类共21种活性成分的含量;根据21种目标成分的含量,用聚类分析(HCA)、主成分分析(PCA)及单因素方差分析(One-way ANOVA)对不同产地连翘药材进行综合评价。结果 HCA结果显示,连翘3个主产区14批药材样品分为2类,第1类为产地山西陵川、陕西合阳、河南辉县、河南林川和河南洛阳,第2类为产地山西安泽和山西古县;PCA结果显示,山西产连翘药材的综合质量优于陕西和河南;One-way ANOVA结果显示,所测成分中,绿原酸、阿魏酸、紫云英苷、牛蒡子苷、木犀草素、连翘酯苷B、连翘酯苷A、松脂醇-β-D-葡萄糖苷、连翘苷和松脂醇10种成分在连翘3个主产区样品间存在显著差异。结论 所构建的多元活性成分同时测定结合多元统计分析的综合评价体系,成功地应用于不同产地连翘药材的质量评价,可为连翘药材内在质量的综合评价和全面控制提供新的方法参考,亦为连翘药材的道地性研究提供基础资料。
Abstract
OBJECTIVE To comprehensively evaluate the quality of Forsythiae Fructus from different habitats based on simultaneous determination of multiple active components combined with multivariate statistical analysis. METHODS Twenty-one bioactive constituents including phenylethanoid glycosides, lignans, flavonoids and phenolic acids in Forsythiae Fructus from different producing areas were simultaneously determined by ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry (UFLC-QTRAP-MS/MS). Based on the contents of 21 target components, hierarchical cluster analysis (HCA), principal component analysis (PCA) and One-way analysis of variance (One-way ANOVA) were further employed for the quality assessment of Forsythiae Fructus from different habitats. RESULTS The HCA results showed that 14 batches of medicinal materials from three major producing areas were divided into two categories: the first category was Lingchuan in Shanxi, Heyang in Shaanxi, Huixian in Henan, Linchuan in Henan and Luoyang in Henan; the second category was Anze in Shanxi and Guxian in Shanxi. The PCA results revealed that the comprehensive quality of Forsythiae Fructus from Shanxi was better than those from Shaanxi and Henan. Additionally, the results of One-way ANOVA indicated that the contents of 10 components, ie chlorogenic acid, ferulic acid, astragalin, arctiin, luteolin, forsythiaside B, forsythiaside A, pinoresinol-4-O-β-D-glucoside, phillyrin and pinoresinol were significantly different among the above three provinces. CONCLUSION The integrated evaluation system based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis is successfully applied to the quality evaluation of Forsythiae Fructus from different habitats. It can provide a new reference method for the quality evaluation and control of Forsythiae Fructus, which can also provide basic information for further expounding the geo-authentic of Forsythiae Fructus.
关键词
连翘 /
多元活性成分 /
超快速液相色谱-三重四极杆/线性离子阱质谱法 /
同时测定 /
多元统计分析
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Key words
Forsythiae Fructus /
multiple bioactive constituents /
UFLC-QTRAP-MS/MS /
simultaneous determination /
multivariate statistical analysis
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中图分类号：
R932
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参考文献
[1] Ch.P (2015). Vol Ⅰ(中国药典2015 年版.一部) [S]. 2015: 170-171.
[2] WANG Z Y, XIA Q, LIU X, et al. Phytochemistry, pharmacology, quality control and future research of Forsythia suspensa (Thunb.) Vahl: a review[J]. J Ethnopharmacol, 2018, 210: 318-339.
[3] GUO H, LIU A H, LI L, et al. Simultaneous determination of 12 major constituents in Forsythia suspensa by high performance liquid chromatography—DAD method[J]. J Pharm Biomed Anal, 2007, 43(3): 1000-1006.
[4] GUO D D, ZHANG L, ZHU X F. Investigation report on the germplasm resources of Forsythiae Fructus[J]. Lishizhen Med Mater Med Res (时珍国医国药), 2012, 23(10): 2601-2603.
[5] WU T, WEI S, MI L H, et al. DNA fingerprint library construction and cluster analysis of Forsythia suspensa from different origins[J]. Chin Tradit Herb Drugs (中草药), 2016, 47(5): 816-820.
[6] WU Y, WANG H, FANG M F, et al. Review of studies on influence of environmental factors on formation of medicinal materials from botanicals[J]. Nat Prod Res Dev (天然产物研究与开发), 2013, 25(3): 416-420.
[7] NI Y N, ZHUANG H, KOKOT S. A high performance liquid chromatography and electrospray ionization mass spectrometry method for the analysis of the natural medicine, Forsythia Suspensa[J]. Anal Lett, 2014, 47(1): 102-116.
[8] FU Y F, LI Q, BI K S. Determination of seven components in Forsythia suspensa by RP-HPLC[J]. Chin Tradit Herb Drugs (中草药), 2013, 44(8): 1043-1046.
[9] FENG S. Quality evaluation study and variety and quality related research of Forsythiae Fructus based on chemical and biological fingerprint technology [D]. Jinan: Shandong University of Traditional Chinese Medicine, 2014.
[10] WANG S N, HUA Y J, XU L, et al. Simultaneous determination of multiple constituents in Scrophulariae Radix from different habitats and commercial herbs by UPLC-QTRAP-MS/MS[J]. Chin Pharm J (中国药学杂志), 2016, 51(16): 1416-1422.
[11] LI P H, WU Q N, YAN H, et al. Drying processing method for Zingiberis Rhizoma based on multiple bioactive constituents[J]. Chin Tradit Herb Drugs (中草药), 2018, 49(18): 4293-4301.
[12] JIANG H, DING H P, BAI H J. Comprehensive assessment of quality characteristics of introduced table jujube cultivars in Southern Xinjiang[J]. Food Sci (食品科学), 2016, 37(3): 55-59.
[13] DAN H L, MENG J. Variable selection for complex industrial control system based on principal component analysis[J]. J Chin Ceram Soc (硅酸盐学报), 2002, 30(S1): 131-134.
[14] TAN M X, CHEN J L, ZOU L S, et al. Effects of different drying methods on multiple bioactive constituents of Ophiopogonis Radix[J]. Nat Prod Res Dev (天然产物研究与开发), 2019, 31(1): 100-109.
[15] WANG Z. Analytical method and strategy for discovery, identification and determination of target multiple compounds in complex matrix samples [D]. Beijing: Peking Union Medical College, 2016.
[16] HU M Z, MENG X S, WANG C, et al. Advances in application of mass spectrometry in high-throughput and rapid detection[J]. J Instrum Anal (分析测试学报), 2018, 37(2): 127-138.
[17] PENG L, WANG Y Z, ZHU H B, et al. Fingerprint profile of active components for Artemisia selengensis Turcz by HPLC-PAD combined with chemometrics[J]. Food Chem, 2011, 125(3): 1064-1071.
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脚注
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基金
江苏高校优势学科建设工程项目资助(YSXK-2014);江苏高校品牌专业建设工程项目资助(PPZY2015A070)
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